cort treatment  (Sino Biological)

Journal: Cell discovery

Article Title: Psychological stress-induced systemic corticosterone directly sabotages intestinal stem cells and exacerbates colitis.

doi: 10.1038/s41421-025-00796-y

Figure Lengend Snippet: Fig. 5 FKBP5 is upregulated by corticosterone in ISCs. a Schematic diagram of RNA-seq analysis of ISCs isolated from the small intestine of Ctrl, restraint stress-treated, vehicle-treated and CORT-treated mice. Lgr5-EGFP-IRES-creERT2 mice were exposed to restraint stress or CORT treatment for 1 day (n = 3). The small intestinal crypt fractions were isolated and dissociated into single-cell suspensions. ISCs were sorted by using flow cytometry and subjected to RNA-seq analysis. b Heatmap showing the expression of stem cell marker genes, Wnt-related genes and proliferation-related genes in ISCs isolated from the small intestine of Ctrl, restraint stress-treated, vehicle-treated and CORT-treated mice. c Gene set enrichment analysis (GSEA) of transcriptome profiles showing high enrichment of FoxO signaling pathway-related genes in ISCs from the stressed (vs. Ctrl) and CORT-treated (vs. vehicle) mice. Differentially expressed gene, > 2-fold; Padj value < 0.05. d Venn diagram showing overlapping upregulated genes in ISCs from the stress-treated (vs. Ctrl) and CORT-treated (vs. vehicle) mice. e Top 5 upregulated genes in ISCs from the stress-treated (vs. Ctrl) and CORT-treated (vs. vehicle) mice. f Volcano plots showing the genes differentially expressed in ISCs from the stress-treated (vs. Ctrl) and CORT-treated (vs. vehicle) mice. g RT–qPCR analysis of Fkbp5 expression in the small intestinal crypts from the 1-day restraint stress- and CORT-treated mice. n = 6 and 9 mice for the restraint stressed and CORT-treated groups, respectively. h Western blotting for FKBP5 in the small intestinal crypts from the 2-day stress- and CORT-treated mice. β-actin was used as a loading control. i RT–qPCR analysis of Fkbp5 expression in intestinal organoids after ex vivo treatment with 200 μM CORT or 200 μM CORT simultaneously supplemented with 5 μM RU486 (n = 3). j A chromatin immunoprecipitation assay was performed using an antibody against NR3C1 on crypts isolated from the WT mice treated with vehicle or CORT for 1 day (n = 4). IgG was used as a negative control, and the enrichment of NR3C1 binding to the Fkbp5 promoter was quantified using qPCR. The data are presented as the mean ± SD. The statistical analysis was performed by unpaired two-tailed Student’s t-test for normally distributed data or the Mann‒Whitney test for non-normally distributed data and by one-way ANOVA with Dunnett’s multiple comparisons test or the Kruskal‒Wallis test with Dunn’s multiple comparisons test for non-normally distributed data. ns, p ≥0.05, *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: For CORT treatment, CORT (Med Chem Express, HY-B1618) was dissolved in 20% sulfobutylether-β-cyclodextrin and intraperitoneally injected either once or daily for 3 weeks at a dose of 5 mg/kg.

Techniques: RNA Sequencing, Isolation, Cytometry, Expressing, Marker, Quantitative RT-PCR, Western Blot, Control, Ex Vivo, Chromatin Immunoprecipitation, Negative Control, Binding Assay, Two Tailed Test

Journal: Cell discovery

Article Title: Psychological stress-induced systemic corticosterone directly sabotages intestinal stem cells and exacerbates colitis.

doi: 10.1038/s41421-025-00796-y

Figure Lengend Snippet: Fig. 6 FKBP5-AKT signaling is responsible for the effect of corticosterone on ISCs. a Representative images of day 4 organoids after ex vivo treatment with 200 μM CORT, 0.25 μM SAFit2, or 200 μM CORT supplemented with 0.25 μM SAFit2. SAFit2, a highly selective FKBP5 inhibitor. Scale bar: 100 µm. b, c Quantification of the bud number (b) and surface area (c) of the organoids in a (n = 6). d, e Western blotting for AKT and pAKT (S473) in the small intestinal crypts of the vehicle-treated, CORT-treated (5 mg/kg/day), SAFit2-treated (20 mg/kg/day) and CORT + SAFit2-treated mice (d) and in the small intestinal crypts of the Ctrl + vehicle-treated, stress+vehicle-treated, Ctrl + SAFit2-treated and stress+SAFit2-treated mice (e). Mice were exposed to those treatments for 2 days. β-actin was used as a loading control. f, g Western blotting for FOXO1 in nuclear and cytoplasmic proteins isolated from the small intestinal crypts of the vehicle-treated, CORT-treated, SAFit2-treated and CORT + SAFit2-treated mice (f) and from the small intestinal crypts of the Ctrl + vehicle-treated, stress + vehicle-treated, Ctrl + SAFit2-treated and stress+SAFit2-treated mice (g). Mice were exposed to those treatments for 2 days. Lamin B1 and tubulin were used as nuclear proteins and cytoplasmic protein loading controls, respectively. h RT–qPCR analysis of p130, p21, and Ccnd1 expression in small intestinal crypts from the 1-day vehicle-treated, CORT- treated, SAFit2-treated and CORT + SAFit2-treated mice (n = 3). The data are presented as the mean ± SD. The statistical analysis was performed by one-way ANOVA with Dunnett’s multiple comparisons test. ns, p ≥0.05, *p < 0.05, **p < 0.01.

Article Snippet: For CORT treatment, CORT (Med Chem Express, HY-B1618) was dissolved in 20% sulfobutylether-β-cyclodextrin and intraperitoneally injected either once or daily for 3 weeks at a dose of 5 mg/kg.

Techniques: Ex Vivo, Western Blot, Control, Isolation, Quantitative RT-PCR, Expressing

Journal: bioRxiv

Article Title: The effect of corticosterone on the acquisition of Pavlovian conditioned approach behavior is dependent on sex and vendor

doi: 10.1101/2024.03.20.586009

Figure Lengend Snippet: Lever-cue-directed (a-c) and food-cup-directed (d-f) behaviors during PavCA sessions 1-5 for Experiment 1. Data are shown as mean ± SEM for (a,d) number of contacts, (b,e) probability, or (c,f) latency to approach the lever-cue (left panels) or food-cup (right panels). (b,c) Compared to male rats from Taconic, male rats from Charles River had a greater number of contacts with the lever-cue (effect of vendor: *p=0.006), greater probability to approach the lever-cue (effect of vendor: **p=0.003) and a decreased latency to approach the lever-cue (effect of vendor: ***p=0.002). Administration of CORT enhanced lever-cue contacts selectively in male rats from Charles River (treatment x vendor: p=0.031, post hoc : ***p=0.001). Similarly, relative to vehicle-treated rats, CORT administration selectively decreased food-cup contacts (treatment x vendor: p=0.033, post hoc : **p=0.003), and the probability to approach the food cup (treatment x vendor: p=0.035, post hoc : ***p=0.001) in male rats from Charles River.

Article Snippet: There were no significant effects of CORT treatment on the expression of sign-tracking behavior, but the effects of sex and vendor observed in the absence of treatment were consistent between all three experiments, suggesting that rats from Charles River have a greater tendency to sign-track relative to those from Taconic.

Techniques:

Journal: bioRxiv

Article Title: The effect of corticosterone on the acquisition of Pavlovian conditioned approach behavior is dependent on sex and vendor

doi: 10.1101/2024.03.20.586009

Figure Lengend Snippet: Lever-cue-directed (a-c) and food-cup-directed (d-f) behaviors during PavCA sessions 1-5 for Experiment 2. Data are shown as mean ± SEM for (a,d) number of contacts, (b,e) probability, or (c,f) latency to approach the lever-cue (left panels) or food cup (right panels). (a-c) Female rats from Charles River exhibited a greater number of lever-cue contacts (effect of vendor: **p=0.003), an increased probability to approach the lever-cue (effect of vendor: **p=0.004), and an increased latency to approach the lever-cue (effect of vendor: ***p=0.001) compared to female rats from Taconic. (d-f) Compared to female rats from Taconic, female rats from Charles River exhibited a decreased probability to approach the magazine (effect of vendor: *p=0.037) and an increased latency to approach the magazine (effect of vendor: *p=0.044). Regardless of vendor, administration of CORT in female rats significantly increased the latency to approach the food cup (effect of treatment: p=0.042, significance not shown) and had a tendency to attenuate other food-cup-directed behaviors, while increasing lever-cue-directed behaviors (trending effects not shown).

Article Snippet: There were no significant effects of CORT treatment on the expression of sign-tracking behavior, but the effects of sex and vendor observed in the absence of treatment were consistent between all three experiments, suggesting that rats from Charles River have a greater tendency to sign-track relative to those from Taconic.

Techniques:

Journal: bioRxiv

Article Title: The effect of corticosterone on the acquisition of Pavlovian conditioned approach behavior is dependent on sex and vendor

doi: 10.1101/2024.03.20.586009

Figure Lengend Snippet: Effect of CORT or VEH administration on PavCA index in male rats from Taconic and Charles River in Experiment 1. (a) PavCA index (mean ± SEM) averaged across sessions 4 and 5. Charles River rats displayed greater sign-tracking behaviors than Taconic male rats (effect of vendor: *p=0.025). This effect appears to be primarily driven by increased sign-tracking in Charles River males treated with CORT (trend for vendor x treatment interaction: p=0.074). PavCA index (mean ± SEM) across sessions 1-5 in (b) male Taconic rats and (c) male Charles River rats. Administration of CORT increased the propensity to sign-track in (c) male rats from Charles River compared to their VEH counterparts ( post hoc : **p=0.002) but CORT did not significantly affect the propensity to sign-track in (b) male rats from Taconic.

Article Snippet: There were no significant effects of CORT treatment on the expression of sign-tracking behavior, but the effects of sex and vendor observed in the absence of treatment were consistent between all three experiments, suggesting that rats from Charles River have a greater tendency to sign-track relative to those from Taconic.

Techniques:

Journal: bioRxiv

Article Title: The effect of corticosterone on the acquisition of Pavlovian conditioned approach behavior is dependent on sex and vendor

doi: 10.1101/2024.03.20.586009

Figure Lengend Snippet: Effect of CORT or VEH administration on PavCA index in female rats from Taconic and Charles River in Experiment 2. (a) PavCA index (mean ± SEM) averaged across sessions 4 and 5. Charles River female rats displayed greater sign-tracking behavior than Taconic female rats, regardless of treatment (effect of vendor: ***p=0.001). PavCA index (mean ± SEM) across sessions 1-5 in (b) female Taconic rats and (c) female Charles River rats. Administration of CORT increased the propensity to sign-track in female rats compared to those treated with VEH, regardless of vendor (effect of treatment: *p=0.036). There were no significant vendor x treatment interactions for any of these measures.

Article Snippet: There were no significant effects of CORT treatment on the expression of sign-tracking behavior, but the effects of sex and vendor observed in the absence of treatment were consistent between all three experiments, suggesting that rats from Charles River have a greater tendency to sign-track relative to those from Taconic.

Techniques:

Journal: bioRxiv

Article Title: The effect of corticosterone on the acquisition of Pavlovian conditioned approach behavior is dependent on sex and vendor

doi: 10.1101/2024.03.20.586009

Figure Lengend Snippet: (a) PavCA Index (mean ± SEM) across sessions 1-5 (acquisition) and sessions 6-9 (expression) in Experiment 3. Rats from Charles River displayed greater sign-tracking behaviors compared to rats from Taconic both before (sessions 1-5; effect of vendor: p<0.001) and after treatment (sessions 6-9; effect of vendor: p=0.001). Female rats exhibited greater sign-tracking than male rats before treatment (sessions 1-5; effect of sex: p<0.001) and this sex difference persisted during treatment (sessions 6-9; effect of sex: p<0.001). Administration of systemic CORT on sessions 6-9 (indicated by the black arrows) did not significantly impact PavCA Index. PavCA Index (mean ± SEM) averaged across sessions 4 and 5 for the acquisition (ACQ) phase and averaged across sessions 6-9 for the expression (EXP) phase for (b) male and (c) female rats in Experiment 3. PavCA indices were greater during the expression phase than the acquisition phase (effect of phase: p<0.001). Regardless of treatment or vendor, female rats displayed greater sign-tracking behavior compared to male rats across both phases (effect of sex: p<0.001). Additionally, rats from Charles River displayed greater sign-tracking behavior compared to rats from Taconic across both phases (effect of vendor: p<0.001). There were no significant interactions of vendor, sex, or phase and no main effect or interactions with treatment.

Article Snippet: There were no significant effects of CORT treatment on the expression of sign-tracking behavior, but the effects of sex and vendor observed in the absence of treatment were consistent between all three experiments, suggesting that rats from Charles River have a greater tendency to sign-track relative to those from Taconic.

Techniques: Expressing

Journal: bioRxiv

Article Title: The effect of corticosterone on the acquisition of Pavlovian conditioned approach behavior is dependent on sex and vendor

doi: 10.1101/2024.03.20.586009

Figure Lengend Snippet: (a) Number of nose pokes (mean ± SEM) into the inactive and active ports for male rats from Taconic and Charles River in Experiment 1. All rats exhibited a greater number of nose pokes into the active port relative to the inactive port (effect of port: ***p<0.001). (b) Number of lever-cue contacts (mean ± SEM) for male rats from Taconic and Charles River. Compared to male rats from Taconic, male rats from Charles River had a tendency to exhibit a greater number of lever-cue contacts upon its presentation during the conditioned reinforcement test (trend for effect of vendor: #p=0.062). (c) Incentive value index (mean ± SEM) for male rats from Taconic and Charles River. There were no significant effects of vendor, treatment, or interaction on the incentive value index. Prior systemic CORT treatment did not significantly affect any of the measures during the conditioned reinforcement test.

Article Snippet: There were no significant effects of CORT treatment on the expression of sign-tracking behavior, but the effects of sex and vendor observed in the absence of treatment were consistent between all three experiments, suggesting that rats from Charles River have a greater tendency to sign-track relative to those from Taconic.

Techniques:

Journal: bioRxiv

Article Title: The effect of corticosterone on the acquisition of Pavlovian conditioned approach behavior is dependent on sex and vendor

doi: 10.1101/2024.03.20.586009

Figure Lengend Snippet: (a) Number of nose pokes (mean ± SEM) into the inactive and active ports for female rats from Taconic and Charles River in Experiment 2. All rats exhibited a greater number of nose pokes into the active port relative to the inactive port (effect of port: ***p<0.001). (b) Number of lever-cue contacts (mean ± SEM) for female rats from Taconic and Charles River. Female rats from Charles River exhibited a greater number of lever-cue contacts upon its presentation during the conditioned reinforcement test compared to those from Taconic (effect of vendor: *p=0.013). (c) Incentive value index (mean ± SEM) for female rats from Taconic and Charles River. Charles River female rats had a greater incentive value index compared to Taconic female rats (effect of vendor: **p=0.010). Prior systemic CORT treatment did not significantly affect any of the measures during the conditioned reinforcement test.

Article Snippet: There were no significant effects of CORT treatment on the expression of sign-tracking behavior, but the effects of sex and vendor observed in the absence of treatment were consistent between all three experiments, suggesting that rats from Charles River have a greater tendency to sign-track relative to those from Taconic.

Techniques:

Journal: bioRxiv

Article Title: The effect of corticosterone on the acquisition of Pavlovian conditioned approach behavior is dependent on sex and vendor

doi: 10.1101/2024.03.20.586009

Figure Lengend Snippet: Number of nose pokes (mean ± SEM) into the inactive and active ports for (a) male and (d) female rats from Taconic and Charles River in Experiment 3. Female rats poked more in the active port than males ( post hoc : p<0.001), and rats from Charles River (orange) poked more in active port than those from Taconic (blue) ( post hoc : ***p<0.001). All rats exhibited a greater number of nose pokes into the active port relative to the inactive port (effect of port: p<0.001). Number of lever-cue contacts (mean ± SEM) for (b) male and (e) female rats from Taconic and Charles River. Females exhibited a greater number of lever-cue contacts upon its presentation during the conditioned reinforcement test compared to males (effect of sex: p<0.001). Rats from Charles River contacted the lever-cue more than those from Taconic (effect of vendor: ***p<0.001). Incentive value index (mean ± SEM) for (c) male and (f) female rats from Taconic and Charles River. Charles River rats had a greater incentive value index compared to Taconic rats (effect of vendor: ***p<0.001), and females had a greater incentive value index than males (effect of sex: p<0.001). Prior systemic CORT treatment did not significantly affect any of the measures during the conditioned reinforcement test. There were no significant interactions with vendor or sex on any of the measures.

Article Snippet: There were no significant effects of CORT treatment on the expression of sign-tracking behavior, but the effects of sex and vendor observed in the absence of treatment were consistent between all three experiments, suggesting that rats from Charles River have a greater tendency to sign-track relative to those from Taconic.

Techniques:

Journal: Experimental physiology

Article Title: Regulation of the stress response in rats by central actions of glucocorticoids

doi: 10.1113/expphysiol.2008.045971

Figure Lengend Snippet: Values for the DHB for the 50 and 100% DHB Cort treatment groups were above the high standard in the assay and are not shown. *P < 0.05 compared with the DHB Sham group. +P < 0.05 compared with acute stress.

Article Snippet: We determined that DHB Cort (100%) treatment increased the arterial pressure and plasma glucose responses to novel restraint stress in male Sprague–Dawley rats, while treatment with the same dose of Cort systemically had no effect on the blood pressure response to stress ( Scheuer et al. 2007 ).

Techniques: